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Research protocols typically use 0.25-0.5 mg daily via subcutaneous injection near the wrist for 4-8 weeks

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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1 :5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for one hour at room temperature

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